monoclonal rabbit anti cd36  (Proteintech)

Journal: Regenerative Biomaterials

Article Title: A CD36-targeted aptamer-4-butyl-polyhydroxybenzophenone conjugate with pH-responsive release for liver delivery in MASLD

doi: 10.1093/rb/rbag104

Figure Lengend Snippet: Docking results of SF with PPARG (PDB:3DZY), PPARA (PDB:3SP6) and CD36 (PDB:5LGD).

Article Snippet: The rabbit monoclonal antibodies against CD36 (A5792) and β-actin (A19667) were obtained from ABclonal Technology (Wuhan, China).

Techniques:

Journal: Regenerative Biomaterials

Article Title: A CD36-targeted aptamer-4-butyl-polyhydroxybenzophenone conjugate with pH-responsive release for liver delivery in MASLD

doi: 10.1093/rb/rbag104

Figure Lengend Snippet: Specific binding and internalization of ASC. ( A ) Western blot analysis of CD36 protein expression. ( B ) CD36 protein expression was assessed by immunofluorescence staining. Scale bar, 20 μm. ( C ) Flow cytometric evaluation of ASC binding to L-02 and MCF-7 cells. (Cell Only: Blank cells; NAFLD01: Cy5-labeled aptamer sequence; ASC: Cy5-labeled aptamer-drug conjugate; CRO: Cy5-labeled control aptamer sequence; CASC: Cy5-labeled control aptamer sequence-drug conjugate). ( D ) Laser confocal microscopy imaging of Cy5-labeled ASC and LysoTracker-labeled lysosomes. Scale: 10 μm, observed under a 100× oil immersion objective. PCC was calculated to quantify the fluorescence co-localization between Cy5-labeled ASC and LysoTracker-labeled lysosomes. ( E ) Intracellular SF levels after treatment with free SF or ASC. ( F ) Time-dependent intracellular SF levels in L-02 cells treated with free SF or ASC. ( G ) Time-history relationship of intracellular SF concentrations of the ASC drug administration group. Observe the data for 1–6 h. ( H ) SF concentration in culture medium after SF treatment over time. Data are presented as mean ± SD ( n = 3). ## P < 0.01, ### P < 0.001 vs. HepG2 + PA; ** P < 0.01, *** P < 0.001 vs. L-02 + PA; *** P < 0.001 vs. SF at the same concentration.

Article Snippet: The rabbit monoclonal antibodies against CD36 (A5792) and β-actin (A19667) were obtained from ABclonal Technology (Wuhan, China).

Techniques: Binding Assay, Western Blot, Expressing, Immunofluorescence, Staining, Labeling, Sequencing, Control, Confocal Microscopy, Imaging, Fluorescence, Concentration Assay

Journal: Journal of Translational Medicine

Article Title: 15,16-Dihydrotanshinone I, a novel β-catenin-targeting inhibitor that inhibits its nuclear translocation and reduces downstream CD36 expression in cancer

doi: 10.1186/s12967-025-07317-1

Figure Lengend Snippet: CD36 is a β-catenin downstream signaling molecule in CRC. ( A ) Western blot showing the expression of β-catenin and CD36 in β-catenin-overexpressed DLD-1 cells (DLD-1- β-catenin) and control cells. ( B ) Immunohistochemical (IHC) staining of CD36 in the human colon cancer tissue and normal colon tissues (US Biomax #CO702b). ( C ) Correlation between the survival probability and CD36 expressions in CRC patients. Western blot showing the protein expressions of CD36 in ( D ) DLD-1 cells, ( E ) HT55 cells and ( F ) β-catenin-overexpressed DLD-1 cells (DLD-1- β-catenin) after 48 h DHTS treatments. The relative intracellular ATP levels in ( G ) DLD-1, ( H ) HT55, ( I ) CD36-overexpressed DLD-1 cells (DLD-1-CD36) and ( J ) β-catenin overexpressed DLD-1 cells (DLD-1-β-catenin) after 48 h DHTS treatments. Shown is the mean ± SEM, n = 3 individual experiments, * p < 0.05, ** p < 0.01 compared to control. DHTS, dihydrotanshinone I; DHTS-L, low dosage of dihydrotanshinone I (1.2 µM for DLD-1, 1 µM for HT55); DHTS-H, high dosage of dihydrotanshinone I (1.6 µM for DLD-1, 2 µM for HT55)

Article Snippet: The TMA sections were incubated with a primary monoclonal rabbit anti-CD36 antibody (catalog number 14347, Cell Signaling Technology) in Tris-buffered saline (TBS) containing 1% bovine serum albumin (BSA) for 1 h. After washing with TBS, the sections were incubated with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Bio-Rad).

Techniques: Western Blot, Expressing, Control, Immunohistochemical staining, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: 15,16-Dihydrotanshinone I, a novel β-catenin-targeting inhibitor that inhibits its nuclear translocation and reduces downstream CD36 expression in cancer

doi: 10.1186/s12967-025-07317-1

Figure Lengend Snippet: β-catenin/CD36 axis mediates the anti-CRC effect of the DHTS-laden PLGA-co-PEG nanoparticles in vivo. ( A ) Flowchart showing the preparation of DHTS-laden PLGA-co-PEG nanoparticles (DHTS-NP). ( B ) The dissected tumor tissues of the CRC-bearing xenograft mouse models after DHTS-NP or 5-fluorouracil (5-FU) treatments. ( C ) Tumor volume and ( D ) tumor weight of the dissected tumors after DHTS-NP or 5Fu treatments. ( E ) Western blot showing the nuclear expressions of β-catenin in the xenograft tissues. Western blot showing the expressions of ( F ) cytoplasmic β-catenin, ( G ) LEF-1, TCF-4 and CD36 in the xenograft tissues. Shown is the mean ± SEM, n = 6–8 mice in each group, * p < 0.05, ** p < 0.01 compared to control. DHTS, dihydrotanshinone I; DHTS-NP, DHTS-laden PLGA-co-PEG nanoparticles; DHTS-NP-L (15 mg/kg DHTS); DHTS-NP-H (20 mg/kg DHTS); 5Fu, 5-fluorouracil (10 mg/kg)

Article Snippet: The TMA sections were incubated with a primary monoclonal rabbit anti-CD36 antibody (catalog number 14347, Cell Signaling Technology) in Tris-buffered saline (TBS) containing 1% bovine serum albumin (BSA) for 1 h. After washing with TBS, the sections were incubated with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (Bio-Rad).

Techniques: In Vivo, Western Blot, Control

Journal: Advanced Science

Article Title: Spatial Transcriptomic Study Reveals Heterogeneous Metabolic Adaptation and a Role of Pericentral PPARα/CAR/Ces2a Axis During Fasting in Mouse Liver

doi: 10.1002/advs.202405240

Figure Lengend Snippet: Enhancement of fatty acid oxidation pathways, including mitochondrial and peroxisomal β‐oxidation and microsomal ω‐oxidation, during fasting. A) Upregulated genes involved in the process of lipid metabolism during fasting are highlighted in red. B) Quantified profiles of the indicated genes along the PN‐CV axis from ST. C,D) Quantified profiles of Cd36 C) and Plin5 D) along the PN‐CV axis from ST, accompanied by corresponding qRT‐PCR analysis, visualization in tissue spots, and IHC staining in liver sections.n = 4 mice for each group.Data are shown in mean ± SEM; ns, not significant, *p < 0.05, **p < 0.01, ****p < 0.0001 by Student's t test.

Article Snippet: The following primary antibodies were used: polyclonal Rabbit anti‐Thrsp antibody (1:200, #13054‐1‐AP, Proteintech), polyclonal Rabbit anti‐Gstm3 antibody (1:200, #15214‐1‐AP, Proteintech), monoclonal rat anti‐Cyp51 antibody (1:200, # ab210792, Abcam), polyclonal Rabbit anti‐Gstm3 antibody (1:200, #15214‐1‐AP, Proteintech), polyclonal Rabbit anti‐Bip antibody (1:200, #3177, CST), monoclonal Rabbit anti‐Cd36 antibody (1:200, #ET1701‐24, HUABIO), polyclonal Rabbit anti‐Plin5 antibody (1:200, #26951‐1‐AP, Proteintech), polyclonal Rabbit anti‐Ces1 antibody (1:200, #PA5‐19740, ThermoFisher), polyclonal Rabbit anti‐Ces2 antibody (1:200, #PA5‐102415, ThermoFisher).

Techniques: Quantitative RT-PCR, Immunohistochemistry

Journal: Antioxidants (Basel, Switzerland)

Article Title: Impact of Hydroxytyrosol-Rich Extract Supplementation in a High-Fat Diet on Gilthead Sea Bream ( Sparus aurata ) Lipid Metabolism.

doi: 10.3390/antiox13040403

Figure Lengend Snippet: Figure 5. Representative Western blots and quantification of CD36 protein levels normalized to total protein (RevertTM) in liver samples of gilthead sea bream juveniles fed with the experimental diets. A high-fat diet alone (HF) or supplemented with hydroxytyrosol (HF+HT) (0.52 g HT per kg feed) at standard (ST) (3% total fish weight/tank) or restricted ration (RE) (40% reduction) for 8 weeks. Data are shown as mean + SEM (n = 4 fish). Statistical differences are indicated in three components: diet (D), ration (R) and interaction (D*R), using two-way ANOVA (p < 0.05, shown in bold).

Article Snippet: Following a wash, membranes were blocked with Intercept® Blocking buffer (TBS) (#927-50000, LI-COR Inc., Servicios Hospitalarios, Barcelona, Spain) at room temperature for 1 h and incubated overnight at 4 ◦C with rabbit monoclonal anti-CD36 (D8L9T) primary antibody (1:500, #14347, Cell Signaling Technology, Beverly, MA, USA) diluted in the same blocking buffer-TWEEN® 20 0.1%.

Techniques: Western Blot